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Well-Being and Vitality

That feeling of well-being, vitality and joy that takes place in many occasions, like when sport practices has a biochemical origin, part of which is produced by a called substance endorfina. The endorfinas are peptides (small proteins) derivatives of a precursor produced concerning the hipfisis, a small gland that this located in the base of the brain. When we make sport this gland is stimulated, taking place endorfinas in the organism, those that are going to act on the receivers that cause analgesia, besides producing a similar sedative effect to which it generates morphine, opioide exogenous coarsely known by these same properties. It is by that the endorfinas are considered our opioides endogenous, that is to say, produced by our organism. The studies demonstrate that the endorfinas are able to inhibit the nervous fibers that transmit the pain, besides acting at cerebral level producing subjective experiences, that are intense sensations, known well by the sportsmen as they are the diminution of the anxiety and the well-being sensation. Besides the analgesia and sedation indicated above.

Not only the sport is a stimulus that makes us secretar endorfinas, since in certain situations of stress also its production like in the sexual acupuncture, relations, the suggestion and also in certain ritual or ceremonial dances has been verified. It is difficult to establish what of these activities is the one that better stimulates the production of endorfinas. Nevertheless this demonstrated that the resistance exercises, yes produce a significant increase in the secretion of endorfinas. Numerous studies have demonstrated that after to have produced aerobic physical activity, it exists clearly and significant increase of the endorfinas after the exercise. In runners of long distances it has been managed to revert the analgesia produced by the exercise, administering inhibiting of morphine. What demonstrates that they use the same physiological receivers and in addition the roll of the endorfinas like analgesic is verified in these athletes.

The Plate

The wells of a plate with antibodies or antigens are covered. 2. The samples are added, controls and standards to wells of the plate and are incubated to temperatures that oscillate between room temperature and 37 C, by a period of time determined, according to characteristics of test. During the incubation, a part of the antigen of the sample is united to the antibody of the covering of the plate, or the antibody of the sample is united with the antigen located in the covering of the plate, based on its presence and amount in the analyzed sample. 3.

After the incubation, antigen the united organizations or antibodies are not washed and they retire of the plate, using the ELISA washer that uses a suitable one. 4. Next, a secondary antibody, denominated adds itself which has an enzyme that will react with a substrate to produce a change of color. 5. A second period of incubation begins then, during which the conjugated one will be united to the complex antigen-antibody in wells of the plate. 6. After the incubation, a new washing is realised to retire of wells of the plate any vestige of the not united conjugated one. 7.

A substrate is added. The enzyme will react with the substrate and will cause to a change of color in the solution, offering means to measure conjugated amount of that simultaneously will say how much complex antigen antibody is present. Another period of incubation will allow that this reaction takes place. 8. Turned the time of incubation, a reagent is added to stop the reaction substrate-enzyme and to prevent additional changes with color. Generally this reagent is a diluted acid. 9. Finally, the reading of the plate in the ELISA analyzer takes place. The values of the results are used to determine the amounts of present specific antigen or antibody in the sample.