On the theological level, no longer is the theme of the mission. A peripheral issue And why only the missions were only religious orders? Because I had a concept of mission, ecclesiology, peripherals, optional optional. Only those who were missionaries came to the missionary orders. And the Church was not missionary in nature. However, all theological reflection with the Council, reached its clarity and depth.
Paul VI: a The Church exists to evangelize John Paul II: a The Church of Jesus Christ, or, is missionary, or is not the Church Ad Gentes Jesucristoa : a The Church is missionary by naturalezaa That is the best way, the best thermometer to measure, even our love for Christ and the Church. If you want to know how much you love Christ and the Church, ask yourself: Cuanto have passion for the mission? The greatness of Paul, is its passion for the mission, his passion for the Church, his passion for Jesus Christ. The spread of the gospel to the ends of the earth, that was the passion of Paul. And that was the big thermometer that measured their love for Christ. Missionary vocations .- The Church is made for us.
But know that we are the bride of Christ. What are the body of Christ the Head. In this Church there charisms and ministries. This Church is made up of various vocations. Some are religious, others are diocesan, others are secular, some are married, etc.., Etc. yCuantas are vocations? The general vocations in the life of the Church, because the vocation of the Christian life.
The wells of a plate with antibodies or antigens are covered. 2. The samples are added, controls and standards to wells of the plate and are incubated to temperatures that oscillate between room temperature and 37 C, by a period of time determined, according to characteristics of test. During the incubation, a part of the antigen of the sample is united to the antibody of the covering of the plate, or the antibody of the sample is united with the antigen located in the covering of the plate, based on its presence and amount in the analyzed sample. 3.
After the incubation, antigen the united organizations or antibodies are not washed and they retire of the plate, using the ELISA washer that uses a suitable one. 4. Next, a secondary antibody, denominated adds itself which has an enzyme that will react with a substrate to produce a change of color. 5. A second period of incubation begins then, during which the conjugated one will be united to the complex antigen-antibody in wells of the plate. 6. After the incubation, a new washing is realised to retire of wells of the plate any vestige of the not united conjugated one. 7.
A substrate is added. The enzyme will react with the substrate and will cause to a change of color in the solution, offering means to measure conjugated amount of that simultaneously will say how much complex antigen antibody is present. Another period of incubation will allow that this reaction takes place. 8. Turned the time of incubation, a reagent is added to stop the reaction substrate-enzyme and to prevent additional changes with color. Generally this reagent is a diluted acid. 9. Finally, the reading of the plate in the ELISA analyzer takes place. The values of the results are used to determine the amounts of present specific antigen or antibody in the sample.